when “resetting” the edited FlowJo matrix, remember that it was simply a duplicate of the initial acquisition matrix.To remove the altered compensation matrix from a sample or samples, simply go back to the matrix editor and drag the original (unedited) version of the compensation matrix back on to a sample or group of samples.Changes to the spillover values will update the parameters displayed in the graph window.Any of the values in this new FlowJo derived matrix can continue to be edited and will be applied to the sample(s).Of note while editing an acquisition matrix: We have entered a series of values (1 to 100) in the FITC channel to exemplify this (see image below, top row). The higher the value, the darker the shades of yellow/orange will be applied. The color of the box will change depending on the input value. If you are editing the acquisition- defined matrix, the default name will be “Acquisition-defined-copy” and will be assigned a new color (default is blue).Ĥ) Mouse over and click in a box in the matrix window to edit the existing values. Hit “Enter” or “Return” on the keyboard to finalize the value. Therefore, a copy must be created in FlowJo that can be edited.Ģ) Click “Edit” to make a new (live) matrix and modify any of the matrix spillover values.ģ) A drop- down menu will then ask you if you would like to continue editing the acquisition matrix, click “Yes”.Ī new matrix will be created and immediately applied to all samples in the workspace. Acquisition matrices are usually grey by default.ĭouble-clicking the grid opens the matrix editor :įlowJo does not allow direct editing of the acquisition matrix to maintain the integrity of the FCS standard since these compensation values are stored within the file. If this gird appears when the data are first loaded, this means there is a compensation matrix embedded in the file and FlowJo has applied it. The color grid indicates that a compensation matrix has been applied to the sample. To alter an acquisition matrix on a file, please follow these steps:ġ) Double click on the grid next to the sample. You can also create an entirely new matrix with parameters of your choosing (See “Creating a New Matrix File” below). You can duplicate an existing matrix, which first must be edited and then applied to samples in the workspace.Any changes to spillover values are immediately applied to your samples in the workspace.
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